Abstract

Natural dentin repair is a biologically mediated process that preserves tooth vitality through odontoblast activity and intracellular signaling pathways. This study employed a mixed methods design, combining laboratory experiments with descriptive analytical surveys of dentists in Egypt, to investigate the mechanisms of odontoblast differentiation and assess clinical knowledge and practices related to regenerative dentistry. Laboratory analyses included microscopic and molecular techniques to evaluate cellular signaling during dentin repair, while questionnaires were distributed to 100 dentists across public and private sectors. Data were analyzed using quantitative statistics, qualitative assessments, and integrative interpretation of laboratory and clinical findings. Results revealed significant positive correlations between dentists’ knowledge of intracellular signaling and their clinical practices, with Spearman correlation coefficients ranging from 0.52 to 0.65 (p = 0.01). A majority of respondents (80%) reported experience with mesenchymal stem cell therapy for craniofacial bone remodeling or dental tissue regeneration, and 70% observed improvements in bone density, while 65% reported enhanced dental tissue regeneration. Imaging studies were widely used, with 85% of participants undergoing radiographic or CT evaluations to monitor progress. Despite these encouraging outcomes, only 35% of dentists were familiar with molecular techniques targeting signaling pathways, and just 40% reported applying clinical strategies to enhance odontoblast differentiation. Notably, 75% expressed interest in specialized training to improve their knowledge and practices in regenerative dentistry. The study concludes that while theoretical awareness of dentin repair mechanisms is relatively strong, clinical application remains limited. Bridging this gap through structured training and evidence-based protocols will be essential for integrating molecular insights into routine dental practice, thereby improving dentin repair outcomes and long-term tooth vitality.

Abstract

Periodontal disease is a multifactorial condition characterized by chronic inflammation and progressive destruction of supporting tissues, with significant implications for oral health and systemic well-being. This study investigated the correlation between inflammatory responses and regenerative outcomes in periodontal supporting tissues, emphasizing the role of epigenetic regulation. A mixed methods approach was employed, combining clinical assessments, patient-reported outcomes, and molecular analyses. Spearman correlation analysis revealed strong associations between clinical indicators of inflammation and perceived regenerative improvements, underscoring the interplay between destructive and reparative processes. Demographic and lifestyle factors, including smoking and systemic health conditions, were found to influence variability in responses. Regenerative therapies demonstrated favorable outcomes in terms of clinical attachment gain, alveolar bone support, and patient satisfaction. These findings highlight the importance of integrating epigenetic insights with clinical practice to develop personalized strategies for managing periodontal disease and enhancing tissue regeneration

Abstract

Stem cell therapy has emerged as a promising approach for craniofacial bone remodeling and dental tissue regeneration. In this study, a mixed-methods design was employed, combining laboratory analyses with clinical data from 100 participants. Results demonstrated that 80% of respondents had received mesenchymal stem cell (MSC) therapy, with 70% reporting improvements in bone density and 65% noting enhanced dental tissue regeneration. Imaging tests confirmed progress in 85% of cases, while overall satisfaction with therapy outcomes was reported by 80–85% of participants. Correlation analysis revealed strong positive relationships between stem cell treatment and improvements in bone and dental tissues, with coefficients ranging from 0.60 to 0.70 (p < 0.01). Side effects were minimal, with only 20% reporting complications such as swelling or pain. These findings underscore the therapeutic potential of stem cell-based interventions, highlighting both their efficacy and safety. The study concludes that stem cell therapy represents a viable and effective strategy for regenerative dentistry and craniofacial surgery, though larger multicenter trials are needed to standardize protocols and validate long-term outcomes.

ABSTRACT: 

The current finding has been planned to estimate the biological impacts of the regular administration of 

antioxidant vitamin C on the submandibular gland of aged mice. Material & methods: Thirty aged Wister male 

mice (12-15 months), weighing 300 to 350gs, were utilized in this research. The mice were separated randomly 

into double groups (15 mice/each). The firstgroup where animals administrated distilled water orally. The 

second group where animals administrated orally vitamin C about 100mg once daily for a month by using the 

syringe. After a month all mice were sacrificed.The samples were attained from the gland of the mice in the 

whole group. After that, the slides were prepared and observed by the microscope. Results: Histopathological 

findings in the group1 included; damage of parenchymal and connective tissues of glands. In group2, the acini 

and ducts appeared approximately normal. Ultra-structure inspection of the group1 showed some vacuoles in 

the parenchyma, as well as, distorted most organelles. In group2, the ultra-structural manifestations appeared 

nearly normal. Conclusion: Vitamin C revealed obvious improvement toreduce aging alterations of the salivary 

gland.

KEYWORDS: Histopathological and ultrastructural effects, Antioxidant Vitamin C, Submandibular salivary 

gland, Aged mice

Abstract: Objective: The present study has been designed to evaluate the histological effects of the daily consumption of omega-3 on submandibular salivary gland of old rats. Material & methods: Forty old white male rats (12-15 month age old), weighing 300 to 350gs, were used in this study. The rats were divided into two groups (20 rats/each). Control group where rats did not receive any dietary supplement. Experimental group where rats received omega-3 (60 mg/Kg) twice daily for three months through intra- gastric intubation. All rats were sacrificed after three months. Soft tissue specimens were obtained from submandibular salivary gland of the rats in all the studied groups. The sections were examined histologically and ultra-structurally. Results: Histopathological changes observed in control group included; distortion and decrease in the overall size of the acini and granular convoluted tubules increase in the amount of fatty tissue, fibrous tissue and inflammatory cell infiltration. In experimental group, the acini and ducts appeared nearly normal and there was marked decrease in the inflammatory cell infiltration. Electron microscopic examination of the control group revealed cytoplasmic vacuolization in acini and ducts, degenerated organelles and pyknotic nuclei. In experimental group, the acini and ducts appeared nearly normal in size, shape and structure. Conclusion: Omega-3 showed significant improvement in many age related changes of submandibular salivary gland.الملخص: الهدف: صممت الدراسة الحالية لتقييم التأثيرات النسيجية لليوم استهلاك أوميغا 3 على الغدد اللعابية تحت الفك السفلي للفئران القديمة. المواد والطرق: أربعون ذكرا أبيض يبلغ من العمر تم استخدام الفئران (12-15 شهرا) ، التي تزن 300 إلى 350 جراما ، في هذه الدراسة. تم تقسيم الفئران إلى مجموعتين (20 جرأ / لكل منهما). المجموعة الضابطة حيث لم تتلق الفئران أي مكمل غذائي. المجموعة التجريبية حيث الفئران تلقى أوميغا 3 (60 مجم / كجم) مرتين يوميا لمدة ثلاثة أشهر من خلال التنبيب داخل المعدة. تم التضحية بجميع الفئران بعد ثلاثة أشهر. تم الحصول على عينات الأنسجة الرخوة من الغدة اللعابية تحت الفك السفلي للفئران في جميع المجموعات المدروسة. تم فحص الأقسام من الناحية النسيجية والهيكلية الفائقة. النتائج: نسيجية مرضية وشملت التغييرات التي لوحظت في المجموعة الضابطة; تشويه وانخفاض في الحجم الكلي للأسيني والحبيبات تزداد الأنابيب الملتوية في كمية الأنسجة الدهنية والأنسجة الليفية وتسلل الخلايا الالتهابية. في المجموعة التجريبية ، بدت الأسيني والقنوات طبيعية تقريبا وكان هناك انخفاض ملحوظ في الالتهابات تسلل الخلايا. كشف الفحص المجهري الإلكتروني للمجموعة الضابطة عن تفريغ السيتوبلازمي في أسيني والقنوات والعضيات المتدهورة والنوى pyknotic. في المجموعة التجريبية ، ظهرت الأسيني والقنوات تقريبا طبيعي في الحجم والشكل والهيكل. الخلاصة: أظهرت أوميغا 3 تحسنا كبيرا في العديد من الأمراض المرتبطة بالعمر تغيرات الغدة اللعابية تحت الفك السفلي.